Training

Training

Genewin offers a wide of range of fields for student training programs and projects.

  • Soil analysis – Estimation of nutrients present in soil and identification of deficiencies
  • Water analysis – Estimation of parameters in water
  • Leaf analysis – Estimation of nutrients present in soil and identification of diseases and deficiencies
  • Plant Tissue Culture techniques
  • Explant Initiation of plants through Tissue culture
  • Production of Biofertilizers using carriers
  • Formulation of Biopesticides against common plant pests

Module 1: Basic molecular Biology techniques

  • Isolation of DNA
  • RNA extraction
  • Isolation of Genomic DNA
  • Agarose Gel Electrophoresis
  • DNA Purification by gel elution
  • Quantification of DNA and RNA by UV spectrophotometry
  • Determination of melting temperature
  • Restriction digestion of DNA

Module 2:Plant Tissue Culture

  • Media and buffer preparations
  • Sterilization of explants
  • Micropropagation
  • Callus culture
  • Anther culture
  • Synthetic seed production and cell suspension
  • Plant genomic DNA isolation
  • Protoplast isolation

Module 3:Cloning and Expression

  • Bacterial transformation
  • Restriction digestion of vector
  • Competent cell preparation
  • Screening for recombinants
  • Ligation
  • Gel elution of DNA
  • Preparation of Insert
  • Isolation of Plasmid Vector and insert DNA

Module 4:Immunology

  • Ouchterlony Double diffusion
  • Radial immuno diffusion
  • Immuno electrophoresis
  • Rocket immuno electrophoresis
  • Counter current immuno electrophoresis
  • Latex agglutinations and haem agglutination
  • Dot Elisa
  • WIDAL

Module 5:PCR based techniques

  • PCR amplification
  • PCR product amplification
  • RAPD and RFLP fingerprinting
  • Gene specific PCR
  • Restriction Mapping
  • Restriction Digestion of Genomic DNA
  • Sequencing of RNA and DNA

Module 6:Blotting techniques

  • Immunoblotting
  • Southern Blotting
  • Northerrn Blotting
  • Western Blotting
  • DNA Probe labeling and hybridization

Module 7:Bioprocess

  • Aerobic and Anaerobic fermentation
  • Secondary metabolite production
  • Biofuels

Module 8:Bioanalytical techniques in enzymes

  • Extraction and purification of enzymes
  • Quantitative and qualitative analysis of enzymes
  • Enzyme kinetics
  • Enzyme immobilization
  • Assays of clinically important enzymes
  • SDS PAGE and Western Blotting
  • Chromatographic technique
  • Electrophoretic technique
  • Colorimetric technique qualitative analysis of compounds
  • Spectrophotometric and centrifugation techniques
  • Phytochemical analysis Quantitative analysis of compounds

Wet lab-real time projects are also offered.

List of Instruments used in Genewin Biotech

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